Everyone Can Play A Part In The Recovery Journey

The transcript level of unc-31 was increased in mir-71(lf) worms, compared with that of wild-type controls that were normalized to the value of 1. MiR-71 represses the expression of age-1 and unc-31 through the actions on their 3′UTR, but miR-71 is not required for arresting M cell division during L1 diapause. We found that the poor survival rate of daf-16(mu86)(lf) was further decreased by mir-71(lf) (Fig. 2C), consistent with the notion that a portion of miR-71 activities regulate genes that act in parallel to UNC-31–mediated InsR/PI3K signaling for long-term survival during L1 diapause. (B) The severely reduced survival rate of the mir-71(lf) mutant was suppressed by a null allele of unc-31(e928).

Playing The Tape Forward: A Foundation For My Sobriety

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  • Elegans Genetic Center for many mutants of miRNA and other genes.
  • This is consistent with hbl-1 being one of the downstream targets of miR-71, although this modest effect alone is not expected to account for the vulval developmental phenotype in mir-71 mutant.
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  • The LAB tone curve is mostly me experimenting to see how it affects color saturation, particularly in the Blue and Magenta spectrum, which I often adjust.
  • Consistent with the observation described above, the 4-d–starved mir-71(lf) mutants recovering on the RNAi control plates displayed the highly penetrant retarded defect in VPC division.
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